Blar i forfatter "Fantham, Marcus J."

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    • OptiJ: Open-source optical projection tomography of large organ samples 

      Vallejo Ramirez, Pedro P.; Zammit, Joseph; Vanderpoorten, Oliver; Fergus, Riche; Blé, Francois-Xavier; Zhou, Xiao-Hong; Spiridon, Bogdan; Valentine, Christopher; Spasov, Simeon E.; Oluwasanya, Pelumi W.; Goodfellow, Gemma; Fantham, Marcus J.; Siddiqui, Omid; Alimagham, Farah; Robbins, Miranda; Stretton, Andrew; Simatos, Dimitrios; Hadeler, Oliver; Rees, Eric J.; Ströhl, Florian; Laine, Romain F.; Kaminski, Clemens F. (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-10-30)
      The three-dimensional imaging of mesoscopic samples with Optical Projection Tomography (OPT) has become a powerful tool for biomedical phenotyping studies. OPT uses visible light to visualize the 3D morphology of large transparent samples. To enable a wider application of OPT, we present OptiJ, a low-cost, fully open-source OPT system capable of imaging large transparent specimens up to 13 mm tall ...

    • A waveguide imaging platform for live-cell TIRF imaging of neurons over large fields of view 

      Opstad, Ida Sundvor; Ströhl, Florian; Fantham, Marcus J.; Hockings, Colin; Vanderpoorten, Oliver; Tartwijk, Francesca W. van; Qiaojin Lin, Julie; Tinguely, Jean-Claude; Dullo, Firehun Tsige; Kaminski‐Schierle, Gabriele S.; Ahluwalia, Balpreet Singh; Kaminski, Clemens F. (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-02-17)
      Large fields of view (FOVs) in total internal reflection fluorescence microscopy (TIRFM) via waveguides have been shown to be highly beneficial for single molecule localisation microscopy on fixed cells [1,2] and have also been demonstrated for short‐term live‐imaging of robust cell types [3‐5], but not yet for delicate primary neurons nor over extended periods of time. Here, we present a waveguide‐based ...